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Chromatography
- Snigdha Suresh
- 1 day ago
- 1 min read
Chromatography is a technique used to separate a mixture into its components. The mixture is distributed into its components through 2 phases: the stationary and mobile phase.
Stationary and mobile phases
The stationary phase acts as a constraint and slows down the mixture. This phase is also known as the “solid” phase. A porous solid is packed in a glass or metal tube or that constitutes an open tube capillary. The mobile phase moves through this column.
The movement in the mobile phase depends on the interactions between the components and the stationary/mobile phase. The different components distribute themselves according to their relative affinity. The rate of movement of the mixtures varies depending on their solubilities.
Types of chromatography
Some common types of chromatography are
Column chromatography
It separates substances based on differential adsorption of compounds. Column chromatography is used to purify small scale as well as large scale substances. This type of chromatography is used to separate active ingredients.
Paper chromatography
This technique uses paper sheets as the adsorbent through which the stationary phase of the solution passes. This type of chromatography is used to study the process of fermenting and ripening, inspect cosmetics and check the purity of pharmaceuticals.
We use chromatography for various things in our daily life. It is used for ensuring safe and good quality food, pharmaceutical developments, chemical manufacturing process and much more.
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